Regulation of Gibberellin 20-Oxidase and Gibberellin 3β-Hydroxylase Transcript Accumulation during De-Etiolation of Pea Seedlings
نویسندگان
چکیده
منابع مشابه
Changes in gibberellin A(1) levels and response during de-etiolation of pea seedlings.
The level of gibberellin A(1) (GA(1)) in shoots of pea (Pisum sativum) dropped rapidly during the first 24 h of de-etiolation. The level then increased between 1 and 5 d after transfer to white light. Comparison of the metabolism of [(13)C(3)H] GA(20) suggested that the initial drop in GA(1) after transfer is mediated by a light-induced increase in the 2beta-hydroxylation of GA(1) to GA(8). A c...
متن کاملSites of gibberellin biosynthesis in pea seedlings.
Potential sites of gibberellin biosynthesis in 10-day-old ;Alaska' pea (Pisum sativum L.) seedlings were investigated using a cell-free ezyme system capable of incorporating [(14)C]-mevalonic acid into ent-kaurene. In peas, ent-kaurene is assumed to be a committed intermediate in the gibberellin biosynthetic pathway. Comparative results from enzyme assays using extracts from shoot tips, leaf bl...
متن کاملRegulation of transcript levels of a potato gibberellin 20-oxidase gene by light and phytochrome B.
Up to three gibberellin (GA) 20-oxidase genes have now been cloned from several species including Arabidopsis, bean (Phaseolus vulgaris), and potato (Solanum tuberosum). In each case the GA 20-oxidase genes exhibit different patterns of tissue expression. We have performed extensive northern analysis on one of the potato GA 20-oxidase genes (StGA20ox1), which is the only one that shows signific...
متن کاملFeedback control and diurnal regulation of gibberellin 20-oxidase transcript levels in potato.
Tuber formation in potato (Solanum tuberosum) is promoted by short photoperiods and is inhibited by gibberellins (GAs). Endogenous levels of GA1 were shown to decrease in stolons and leaves of potato plants induced to tuberize, which suggests that photoperiodic regulation of GA biosynthesis may play a role in tuber induction. We report the isolation of three potato cDNA clones (StGA20ox1-3) enc...
متن کاملAuxin regulation of the gibberellin pathway in pea.
The auxin indole-3-acetic acid (IAA) has been shown to promote the biosynthesis of the active gibberellin (GA(1)) in shoots of pea (Pisum sativum). We used northern analysis to investigate the timing of IAA-induced changes in transcript levels of PsGA3ox1 (Mendel's LE), PsGA2ox1, PsGA2ox2, and PsGA20ox1, key genes for the later stages of GA(1) biosynthesis and metabolism in pea. Rapid (2-4 h) c...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1999
ISSN: 1532-2548,0032-0889
DOI: 10.1104/pp.121.3.783